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Related Experiment Videos

Complex interactions between yeast TFIIIB and TFIIIC

N Chaussivert1, C Conesa, S Shaaban

  • 1Service de Biochimie et Génétique Moléculaire, CEA-Saclay, Gif sur Yvette, France.

The Journal of Biological Chemistry
|June 23, 1995
PubMed
Summary
This summary is machine-generated.

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General transcription factor TFIIIB70 is key for yeast RNA polymerase III transcription initiation. It interacts with TATA-binding protein and TFIIIC, with intramolecular interactions masking functional domains.

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Transcription Regulation

Background:

  • Yeast class III gene transcription involves sequential assembly of transcription factors TFIIIC, TFIIIB, and RNA polymerase III.
  • The 70-kDa subunit of TFIIIB (TFIIIB70) is a crucial component, mediating interactions within the transcription initiation complex.

Purpose of the Study:

  • To elucidate the specific interactions of TFIIIB70 with TATA-binding protein and TFIIIC (factor tau).
  • To map the protein domains involved in these interactions and investigate the role of intramolecular interactions.

Main Methods:

  • Two-hybrid system used to map protein-protein interaction domains.
  • Analysis of interactions between TFIIIB70, TATA-binding protein, and TFIIIC subunits (tau 131).

Related Experiment Videos

Main Results:

  • TATA-binding protein interacts with the carboxyl-terminal region of TFIIIB70.
  • TFIIIB70 interacts with the tau 131 subunit of TFIIIC.
  • Intramolecular interactions were found to mask functional domains in both TFIIIB70 and tau 131.

Conclusions:

  • TFIIIB70 plays a central role in organizing the transcription initiation complex for yeast class III genes.
  • Understanding these interactions provides insight into the regulation of transcription initiation.
  • Masking of functional domains by intramolecular interactions is a regulatory mechanism in this process.