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Related Experiment Videos

Peptide amidating activity and gastrin processing in the developing sheep pancreas

M Kapuscinski1, A Shulkes

  • 1Department of Surgery, University of Melbourne, Austin Hospital, Victoria, Australia.

The Journal of Endocrinology
|April 1, 1995
PubMed
Summary

This study reveals high levels of peptidylglycine alpha-amidating mono-oxygenase (PAM) and gastrin in fetal sheep pancreas and circulation. PAM activity is not rate-limiting for gastrin bioactivation during development.

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Area of Science:

  • Endocrinology
  • Developmental Biology
  • Gastroenterology

Background:

  • Gastrin regulates gastric acidity and gastrointestinal growth.
  • Gastrin is transiently expressed in the neonatal pancreas.
  • Peptidylglycine alpha-amidating mono-oxygenase (PAM) is crucial for gastrin amide (G-amide) formation.

Purpose of the Study:

  • To investigate the relationship between PAM and gastrin synthesis in the developing ovine pancreas.
  • To measure PAM activity and gastrin concentrations during fetal development.

Main Methods:

  • Measurement of pancreatic PAM activity in ovine fetuses and adults.
  • Quantification of circulating PAM enzyme levels.
  • Assay of pancreatic gastrin (G-amide) and its precursor (G-gly) concentrations.

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Main Results:

  • Pancreatic PAM activity and circulating PAM levels were highest in early fetal stages (95 days gestation) and decreased significantly in adults.
  • Bioactive G-amide and its precursor G-gly concentrations followed a similar pattern, peaking in fetal development.
  • G-gly concentration was consistently less than 10% of G-amide, suggesting efficient processing.

Conclusions:

  • High levels of PAM activity exist in the ovine fetal pancreas and circulation.
  • PAM activity does not appear to be rate-limiting for pancreatic gastrin bioactivation during ovine development.
  • The dual expression of PAM and gastrin in the fetal pancreas mirrors patterns seen in adult peptide-secreting tumors.