Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Molecular characterization of phagosomes

M Desjardins1, J E Celis, G van Meer

  • 1European Molecular Biology Laboratory, Heidelberg, Germany.

The Journal of Biological Chemistry
|December 23, 1994
PubMed
Summary

This study identifies key proteins, including annexins and G proteins, involved in phagosome maturation into phagolysosomes using latex bead uptake. The findings reveal distinct protein and lipid compositions during this crucial cellular process.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluating trastuzumab deruxtecan in patients with gastrooesophageal adenocarcinoma who are ctDNA and HER2 positive: DECIPHER.

ESMO gastrointestinal oncology·2026
Same author

Correction to: The impact of COVID-19 on surgical training: a systematic review.

Techniques in coloproctology·2021
Same author

The quantitative impact of COVID-19 on surgical training in the United Kingdom.

BJS open·2021
Same author

The impact of COVID-19 on surgical training: a systematic review.

Techniques in coloproctology·2021
Same author

Effect of publishing surgeon-specific outcomes on surgical training.

The British journal of surgery·2019
Same author

The effects of human factor related issues on assessors during the recruitment process for general and vascular surgery in the UK.

Annals of the Royal College of Surgeons of England·2019

Area of Science:

  • Cell Biology
  • Immunology
  • Biochemistry

Background:

  • Phagosome maturation into phagolysosomes is essential for cellular defense and involves complex organelle interactions.
  • The molecular machinery governing these interactions remains largely uncharacterized.

Purpose of the Study:

  • To systematically identify proteins and lipids associated with phagosomes during their maturation into phagolysosomes.
  • To utilize latex bead phagosomes for efficient purification and proteomic analysis.

Main Methods:

  • Human and rodent cells were induced to phagocytose latex beads.
  • Phagosomes were purified using discontinuous sucrose gradients.
  • Proteomic analysis was performed using two-dimensional gel electrophoresis and database comparisons.

Related Experiment Videos

  • Lipid composition was analyzed at different stages of phagolysosome formation.

    • Main Results:

    • Identified annexin II, annexin VI, trimeric G protein subunits (beta-1, beta-2), and actin-binding proteins in mouse phagosomes.
    • Observed increased levels of annexin VI on late phagosomes and distinct lipid profiles during maturation.
    • Resolved over 200 polypeptides in human phagosomes, including novel enriched proteins, some not found in current databases.

    Conclusions:

    • Latex bead phagosomes provide a robust system for identifying molecules critical to phagolysosome biogenesis.
    • The study reveals specific protein and lipid dynamics during phagosome maturation, advancing our understanding of this cellular pathway.