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Updated: Aug 19, 2026

In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme
Published on: October 19, 2010
Undermethylated transfer RNA does not support phage RNA-directed in vitro protein synthesis
Abstract:
A cell-free protein synthesis system from Escherichia coli Q13 was depleted of mRNA and tRNA so that restoration of maximum activity was dependent of the addition of these components. Protein synthesis, directed by either MS2 or Qbeta phage RNA, was stimulated significantly by the addition of normal tRNA from either E. coli Q13 or B. In contrast, undermethylated tRNA from methionine-starved E. coli RCrel did not cause this stimulation. It is concluded that undermethylated tRNA Lacks sufficient base modifications to function in protein synthesis.
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