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Related Experiment Videos

Functional and immunoreactive thrombomodulin expressed by keratinocytes

H Mizutani1, T Hayashi, N Nouchi

  • 1Department of Dermatology, Mie University, Faculty of Medicine, Japan.

The Journal of Investigative Dermatology
|December 1, 1994
PubMed
Summary

Human thrombomodulin is expressed by skin keratinocytes, functioning as an anticoagulant and a potential marker for keratinocyte differentiation. This finding highlights thrombomodulin's dual role in epidermal biology.

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Area of Science:

  • Dermatology
  • Hematology
  • Cell Biology

Background:

  • Thrombomodulin (TM) is a key anticoagulant protein primarily found on endothelial cells.
  • Its presence and function in the epidermis are not well understood.

Purpose of the Study:

  • To investigate the expression and function of thrombomodulin in human skin keratinocytes.
  • To determine if epidermal thrombomodulin has anticoagulant properties.

Main Methods:

  • Immunohistopathology using monoclonal antibodies against human thrombomodulin.
  • Western blotting and ELISA on skin and cultured keratinocytes.
  • Functional assays for protein C activation.

Main Results:

  • Thrombomodulin expression was detected in Malpighian-layer keratinocytes, but not basal or upper granular layers.

Related Experiment Videos

  • Full-length thrombomodulin, similar to endothelial TM, was identified in skin and cultured keratinocytes.
  • Epidermal thrombomodulin demonstrated cofactor activity for thrombin-catalyzed protein C activation.
  • Expression was dependent on calcium levels.

    • Conclusions:

    • Human keratinocytes express functional thrombomodulin.
    • Epidermal thrombomodulin may serve as a differentiation marker for keratinocytes.
    • Thrombomodulin contributes to the anticoagulant potential of the skin.