Characterization of antibiotic resistance in Streptococcus suis

Twenty one isolates of Streptococcus suis were screened for antibiotic resistance by growth on antibiotic-containing media, by measuring minimum inhibitory concentrations, by hybridization to specific DNA and oligonucleotide probes for antibiotic resistance genes, and by PCR. The isolates were from a slaughter house survey of respiratory pathogens in Norwegian pigs in 1986. Fifteen isolates were resistant to tetracycline, with MICs ranging from 4-128 micrograms/ml. Genes coding for the Tet O and Tet M determinants were detected in eight and five isolates, respectively. Genes coding for other Gram positive Tet determinants, Tet K, Tet L, and Tet P, were not detected. One isolate was constitutive resistant to erythromycin with MIC of 128 micrograms/ml. Five other isolates carried inducible erythromycin resistance. All these isolates, and five others, were positive in a PCR assay for erythromycin resistance, and hybridized with the Erm C and/or Erm B probes. No resistance against chloramphenicol (5 micrograms/ml) or rifampin (10 micrograms/ml) could be could be detected, but five isolates were resistant to streptomycin (250 micrograms/ml), four isolates were resistant to kanamycin (10 micrograms/ml), and one isolate was resistant to fusicic acid (10 micrograms/ml). In mating experiments with Enterococcus faecalis JH2-2 as recipient, tetracycline, erythromycin, and kanamycin genes were transferred separately to the recipient strain at a rate of 10(-7) transconjugants/recipient cell.