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In vitro platelet function during storage in three different additive solutions

K Koerner1, P Sahlmen, B Zimmermann

  • 1German-Red Cross Blood Bank, Ulm.

Vox Sanguinis
|January 1, 1994
PubMed
Summary
This summary is machine-generated.

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Synthetic media with acetate and gluconate, with or without citrate, effectively preserve platelet quality during storage. These formulations are superior to phosphate-mannitol media and support bacterial growth if plasma is present.

Area of Science:

  • Blood banking and transfusion medicine
  • Biomaterials science
  • Microbiology

Background:

  • Platelet storage requires specialized media to maintain viability and function.
  • Current additive solutions aim to improve platelet preservation beyond traditional plasma storage.

Purpose of the Study:

  • To evaluate the efficacy of three novel synthetic media for platelet storage.
  • To compare the quality of stored platelets in these media against plasma storage.

Main Methods:

  • Platelet concentrates from buffy coats were stored for 5 days in three synthetic media (acetate-gluconate; acetate-gluconate-citrate; phosphate-mannitol) and plasma.
  • In vitro functional tests (aggregation, hypotonic shock response) and metabolic markers (beta-thromboglobulin, platelet factor 4, lactate dehydrogenase, pH) were assessed.

Related Experiment Videos

  • Bacteriological studies were performed to assess microbial growth in the media.

    • Main Results:

    • Platelet aggregation, hypotonic shock response, and release of beta-thromboglobulin, platelet factor 4, and lactate dehydrogenase were comparable between acetate-containing media and plasma.
    • In vitro platelet functions and pH were better preserved in acetate-based media than in the phosphate-mannitol medium.
    • Bacterial growth (Staphylococcus epidermidis) was observed in synthetic media when 20% plasma was carried over.

    Conclusions:

    • Synthetic media containing acetate and gluconate (with or without citrate) offer effective platelet preservation comparable to plasma.
    • Phosphate-mannitol medium resulted in poorer preservation of platelet function and pH.
    • The presence of even small amounts of plasma in synthetic media can support significant bacterial proliferation during platelet storage.