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Related Experiment Videos

Monoclonal antibodies specific for ohmefentanyl

Y F Lu1, C X Tong, A L Wei

  • 1Shanghai Joint Laboratory of Life Sciences, Shanghai Institute of Materia Medica, China.

Zhongguo Yao Li Xue Bao = Acta Pharmacologica Sinica
|July 1, 1994
PubMed
Summary
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Researchers developed two monoclonal antibodies (MAb-D2 and MAb-F4) highly specific for Ohmefentanyl (Ohm). These antibodies function as antagonists, inhibiting Ohm

Area of Science:

  • Pharmacology
  • Immunology
  • Neuroscience

Background:

  • Ohmefentanyl (Ohm) is a potent and selective mu receptor agonist.
  • Development of specific antibodies is crucial for studying opioid receptor interactions.

Purpose of the Study:

  • To generate and characterize monoclonal antibodies (MAbs) specific for Ohmefentanyl.
  • To evaluate the potential of these MAbs as functional antagonists of Ohm.

Main Methods:

  • Hybridoma technology for MAb production.
  • Radioligand binding assays to assess antibody affinity and specificity.
  • Bioassays to evaluate functional antagonism on opioid receptors.

Main Results:

  • Two hybridoma lines (D2 and F4) secreting anti-Ohm MAbs were successfully established.

Related Experiment Videos

  • MAb-D2 and MAb-F4 demonstrated high affinity and specificity for Ohm.
  • These MAbs inhibited [3H] Ohm binding to rat brain opioid receptors and antagonized Ohm's effects on guinea pig ileum contractions.

    • Conclusions:

    • MAb-D2 and MAb-F4 are specific monoclonal antibodies for Ohmefentanyl.
    • These antibodies can serve as valuable tools for functional antagonism of Ohm.