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Related Experiment Videos

Euglobulin lysis time in fresh and stored samples

D Prisco1, R Paniccia, B Bandinelli

  • 1Clinica Medica I. University of Florence, Italy.

American Journal of Clinical Pathology
|December 1, 1994
PubMed
Summary
This summary is machine-generated.

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Storing plasma and euglobulin precipitates at -80°C allows for reliable euglobulin lysis time testing. This method enables simultaneous analysis of multiple samples, improving fibrinolysis research efficiency.

Area of Science:

  • Hematology
  • Clinical Chemistry
  • Thrombosis and Hemostasis

Background:

  • Euglobulin lysis time (ELT) is a key global assay for assessing fibrinolysis.
  • Understanding the impact of sample storage conditions on ELT is crucial for reliable diagnostic and research applications.

Purpose of the Study:

  • To investigate how storage of plasma and euglobulin precipitates affects euglobulin lysis time measurements.
  • To determine optimal storage conditions for preserving the integrity of samples for ELT analysis.

Main Methods:

  • Collected blood from 20 healthy subjects.
  • Measured ELT using fresh samples as reference.
  • Assessed ELT after storing platelet-poor plasma and euglobulin precipitates at -20°C and -80°C for 24 hours.
  • Evaluated ELT with precipitates dissolved in Owren's buffer before freezing.

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Main Results:

  • ELT was significantly prolonged when platelet-poor plasma was stored at -20°C.
  • ELT was significantly prolonged when euglobulin precipitates were dissolved in buffer and stored at -20°C or -80°C.
  • No significant changes in ELT were observed for platelet-poor plasma stored at -80°C or for undissolved precipitates stored at -20°C or -80°C.
  • Results were consistent before and after venous occlusion.

Conclusions:

  • Freezing platelet-poor plasma or euglobulin precipitates at -80°C preserves ELT integrity.
  • Freezing undissolved euglobulin precipitates at -20°C also maintains ELT stability.
  • These storage conditions facilitate batch analysis of samples collected on different days, enhancing laboratory efficiency in fibrinolysis studies.