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Related Experiment Videos

Thrombin receptor expression and function in large granular lymphocyte proliferative disorders

G L Howells1, M Macey, A M Murrell

  • 1Department of Oral Pathology, London Hospital Medical College (LHMC).

British Journal of Haematology
|October 1, 1994
PubMed
Summary
This summary is machine-generated.

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Thrombin receptor is expressed on large granular lymphocytes (LGLs), suggesting a role in LGL biology. Thrombin acts as a chemoattractant for LGLs, potentially recruiting them to inflammatory sites.

Area of Science:

  • Immunology
  • Hematology

Background:

  • Peripheral blood NK-cells and T-cells expressing CD16 and CD56/CD57 also express the platelet-type thrombin receptor.
  • Large granular lymphocytes (LGLs) have T- or NK-cell phenotypes, suggesting thrombin involvement in LGL biology.

Purpose of the Study:

  • To investigate the role of thrombin and its receptor in the biology of large granular lymphocytes (LGLs) in health and disease.
  • To identify and analyze LGL populations expressing the thrombin receptor in proliferative disorders.

Main Methods:

  • Screening patients with LGL proliferative disorders for enriched LGL populations expressing the thrombin receptor.
  • Flow cytometry to analyze thrombin receptor expression on LGLs.
  • Northern analysis to assess mRNA expression of the thrombin receptor.

Related Experiment Videos

  • Chemotaxis assays to evaluate thrombin's effect on LGLs.

    • Main Results:

    • Thrombin receptor expression on LGLs varied from 3% to 86% in polyclonal and clonal disorders.
    • High mRNA expression of the thrombin receptor was observed in a clonal LGL expansion.
    • Thrombin demonstrated chemotactic activity for LGLs from a polyclonal expansion with high receptor positivity.

    Conclusions:

    • Thrombin receptor is present on a significant proportion of LGLs, particularly in proliferative disorders.
    • Thrombin acts as a chemoattractant for LGLs, indicating a potential role in inflammatory site recruitment.
    • These findings suggest thrombin's involvement in LGL biology and potential therapeutic targeting.