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Determination of sulphanilamide in milk by first-derivative and second-derivative spectrofluorimetry
M C Mahedero1, F Salinas, J J Aaron
1Department of Analytical Chemistry, Faculty of Sciences, University of Extremadura, Badajoz, Spain.
Abstract:
First-derivative and second-derivative spectrofluorimetric methods have been developed for the analysis of sulphanilamide (SAN) in milk, using an ethanol-water (60:40, v/v) medium. Linear calibration plots were obtained over a concentration range of 30-120 ppb, with correlation coefficients greater than 0.997. Relative standard deviations were within the range 1.6-3.6%. Limits of detection were 0.7-2.7 ppb. Recoveries of 90-102% were obtained. The derivative fluorescence methods are simple, rapid and sensitive and there is no interference from fluorescent impurities in the determination of low levels of SAN in milk.