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Rapid method for identification of gram-negative, nonfermentative bacilli

L A Otto, M J Pickett

Journal of Clinical Microbiology

|June 1, 1976

Summary

A new rapid oxidative attack (OA) system quickly identifies Gram-negative, nonfermentative bacilli (NFB). This method accurately identifies 99% of NFB strains within 48 hours, improving bacterial identification.

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Area of Science:

Microbiology
Clinical Diagnostics
Bacteriology

Background:

Gram-negative, nonfermentative bacilli (NFB) present identification challenges.
Accurate and rapid identification of NFB is crucial for clinical and research settings.

Purpose of the Study:

To develop and evaluate a rapid oxidative attack (OA) system for the identification of NFB.
To compare the efficacy of the OA method with traditional identification techniques.

Main Methods:

Assayed 112 NFB strains from 25 species using the OA method, buffered single substrate, and oxidative/fermentative methods.
Utilized 38 diverse substrates including organic acid salts, nitrogen compounds, alcohols, and carbohydrates.
Evaluated identification rates, reproducibility, and correlation with established methods.

Main Results:

The OA method achieved 94% identification of NFB strains within 24 hours and 99% within 48 hours.
Demonstrated high reproducibility (99%) and strong correlation with buffered single substrate (98%) and oxidative/fermentative methods (90%).
Presented comprehensive biochemical profiles and identified key tests for NFB identification.

Conclusions:

The rapid oxidative attack (OA) system provides an efficient and accurate method for NFB identification.
This system significantly reduces identification time compared to conventional methods.
The OA method is a valuable tool for routine diagnostic laboratories and research.

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