Related Experiment Videos
The elimination of O-linked glycans from glycoproteins under non-reducing conditions
C A Cooper1, N H Packer, J W Redmond
1School of Chemistry, Macquarie University, North Ryde, NSW, Australia.
Glycoconjugate Journal
|April 1, 1994
Abstract:
A simple procedure is described for the elimination of O-linked glycans from bovine submaxillary mucin under non-reducing conditions, using triethylamine in aqueous hydrazine. The glycans were isolated as the hydrazones, which were converted to the reducing glycans by exchange with acetone in neutral aqueous solution. The glycan alditols obtained after reduction corresponded to those obtained by the reductive beta-elimination of O-glycans.