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Brain ECTO-Mg-ATPase is not the neural cell adhesion molecule
J G Stout1, A Brittsan, T L Kirley
1Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, Ohio 45267-0575.
Biochemistry and Molecular Biology International
|August 1, 1994
Summary
This study investigated the brain ecto-Mg-ATPase and neural cell adhesion molecule (NCAM). Results show ecto-Mg-ATPase is not associated with NCAM in rat and chicken brains.
Area of Science:
- Neuroscience
- Biochemistry
Background:
- The ecto-Mg-ATPase and neural cell adhesion molecule (NCAM) are proteins found in the brain.
- Previous research suggested a potential association between these two molecules.
Purpose of the Study:
- To investigate the relationship between ecto-Mg-ATPase and NCAM in brain tissue.
- To determine if ecto-Mg-ATPase is identical to or closely associated with NCAM.
Main Methods:
- Solubilization and partial purification of rat and chicken brain microsomes using anion exchange chromatography.
- Assay of Mg-ATPase activity and Western blot analysis for ATPase and NCAM proteins.
- Immunoprecipitation of solubilized rat brain microsomes using an anti-NCAM antibody.
Main Results:
- Mg-ATPase activity was separated from NCAM during anion exchange chromatography in both rat and chicken brain preparations.
- Immunoprecipitation with an anti-NCAM antibody did not co-precipitate Mg-ATPase activity, but did precipitate NCAM.
- These findings indicate a lack of association between the two molecules.
Conclusions:
- The brain ecto-Mg-ATPase is neither identical to nor closely associated with the neural cell adhesion molecule (NCAM).
- This study refutes a previous report suggesting a close relationship between ecto-Mg-ATPase and NCAM.