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The human hepatoma cell line, HepG2, secretes functional cholinesterase
J Osada1, J López-Miranda, J Sastre
1Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad de Zaragoza, Spain.
Biochemistry and Molecular Biology International
|August 1, 1994
Abstract:
Confluent monolayers of the human hepatoblastoma-derived cell line, HepG2, were incubated in serum-free medium. This medium was harvested and concentrated. Catalytic activity and immunoblotting of concentrated medium revealed the presence of a functional cholinesterase. This cell line may be an useful model for investigating the regulation and physiological role of the enzyme secreted by liver.