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Univalent-cation-elicited acidification by yeasts

A Kotyk1, G Georghiou

  • 1Institute of Physiology, Prague 4, Czech Republic.

Biochemistry and Molecular Biology International
|August 1, 1994
PubMed
Summary

Univalent cations like potassium significantly boost proton (H+) extrusion in yeast, enhancing acidification of the external medium. This effect is linked to ATP production, not the H+-ATPase or membrane potential.

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Area of Science:

  • * Yeast Physiology and Metabolism
  • * Ion Transport Mechanisms
  • * Cellular Bioenergetics

Background:

  • * Yeast cells actively regulate their internal environment, including pH.
  • * Proton (H+) extrusion is a key process in maintaining cellular homeostasis and energy metabolism.
  • * The role of univalent cations, particularly potassium (K+), in modulating H+ transport is not fully understood.

Purpose of the Study:

  • * To investigate the impact of univalent cations on the rate of H+ extrusion in different yeast species.
  • * To elucidate the relationship between cation-induced H+ extrusion and cellular energy production (ATP).
  • * To determine if the H+-ATPase or plasma membrane potential are involved in this phenomenon.

Main Methods:

  • * Measurement of external medium acidification rates in Saccharomyces cerevisiae, Schizosaccharomyces pombe, and Lodderomyces elongisporus.
  • * Utilized galactose-induced cells and H+-ATPase-deficient mutants.
  • * Monitored H+ extrusion across the entire yeast growth curve.

Main Results:

  • * Addition of univalent cations, especially K+, dramatically increased H+ extrusion rates in all tested yeast species.
  • * Observed increases in H+ extrusion were up to 20-fold higher compared to conditions without K+.
  • * The cation effect on H+ extrusion was independent of H+-ATPase activity and plasma membrane electrical potential, but correlated with ATP production.

Conclusions:

  • * Univalent cations, particularly K+, act as potent stimulators of proton extrusion in sugar-metabolizing yeasts.
  • * This cation-dependent H+ extrusion is primarily driven by metabolic reactions generating ATP, rather than direct H+-ATPase function.
  • * The findings highlight a novel mechanism linking cation availability to cellular acid extrusion in yeast.

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