Related Experiment Videos
[Application of confocal laser microscopy in studying the effect of epidermal growth factor on the seminiferous
F F Rommerts1, R Sanders, A Draaijer
1Erasmus University Rotterdam, Department of Endocrinology and Reproduction, The Netherlands.
Contraception, Fertilite, Sexualite (1992)
|October 1, 1994
Abstract:
Effects of epidermal growth factor (EGF) on pH transients in aggregates of Sertoli cells and germinal cells have been investigated with confocal microscopy using a fluorescent pH sensitive indicator. In some Sertoli cells EGF caused a rapid rise in the pH whereas other Sertoli cells did not respond to EGF. Some Sertoli cells showed a delayed response which coincidated with a similar pH change in neighbouring germinal cells. Since isolated germinal cells never showed a pH response after exposure to EGF, we have concluded that some Sertoli cells may communicate with germinal cells via gap junctions.