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Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
Published on: May 6, 2019
Stimulation of cytolytic activity by interleukin-10
M A Schwarz1, L D Hamilton, L Tardelli
1Immunology Department, Schering-Plough Research Institute, Kenilworth, New Jersey 07033.
Journal of Immunotherapy with Emphasis on Tumor Immunology : Official Journal of the Society for Biological Therapy
|August 1, 1994
Summary
Interleukin-10 (IL-10) enhances anti-tumor immunity by boosting the cancer-killing activity of immune cells. This cytokine shows potential for novel cancer therapies.
Area of Science:
- Immunology
- Molecular Biology
- Cancer Research
Background:
- Interleukin-10 (IL-10) is known for inhibiting cytokine synthesis, including IL-2, IFN-gamma, and TNF-alpha.
- IL-10 modulates various immune activities, but its role in inducing direct cytolytic activity is less understood.
Purpose of the Study:
- To investigate the capacity of IL-10 to induce non-major histocompatibility complex-restricted cytolytic activity in human peripheral blood mononuclear cells (PBMCs).
- To explore the potential of IL-10 as an immunotherapeutic agent against cancer.
Main Methods:
- Human PBMCs were incubated with IL-10 and then used as effector cells in 51Cr release assays against human tumor cell lines.
- Cytotoxicity assays were performed to measure the lytic activity induced by IL-10.
- Neutralization studies using monoclonal antibodies against IL-10, IFN-gamma, and TNF-alpha were conducted.
Main Results:
- IL-10 dose-dependently stimulated or potentiated lytic activity against several human tumor cell lines.
- The observed cytolytic activities were specifically neutralized by anti-IL-10 antibodies, not by antibodies against IFN-gamma or TNF-alpha.
- IL-10 augmented IL-2 or IFN-alpha-induced cytolytic activity and alleviated IL-4-mediated inhibition of IL-2-driven lymphokine-activated killer (LAK) activity.
Conclusions:
- IL-10 can induce and enhance anti-tumor cytolytic activity in human PBMCs.
- IL-10 demonstrates potential as a therapeutic agent for cancer treatment, possibly by enhancing immune cell-mediated tumor cell killing.
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