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Cellular and soluble CD4 measurements in cynomolgus monkeys
Jikken Dobutsu. Experimental Animals
|October 1, 1994
Summary
Researchers successfully labeled T-lymphocytes in Cynomolgus monkeys using anti-human CD4 monoclonal antibodies. Methods included magnetisable bead separation and immunogold labeling, alongside soluble CD4 assays in monkey sera.
Area of Science:
- Immunology
- Primate Research
- Biotechnology
Background:
- CD4 T-lymphocytes play a crucial role in immune responses.
- Cynomolgus monkeys are valuable models for human disease research.
- Accurate labeling of immune cells is essential for immunological studies.
Purpose of the Study:
- To establish reliable methods for labeling T-lymphocytes in Cynomolgus monkeys.
- To investigate the utility of anti-human CD4 monoclonal antibodies in non-human primates.
- To develop assays for soluble CD4 in primate sera.
Main Methods:
- Application of anti-human CD4 monoclonal antibodies for T-lymphocyte labeling.
- Utilizing magnetisable bead separation combined with immunogold labeling.
- Employing an assay for soluble CD4 detection in serum samples.
Main Results:
- Successful labeling of T-lymphocytes in Cynomolgus monkeys was achieved.
- Two distinct labeling methodologies proved effective.
- Soluble CD4 levels were successfully assessed in the monkeys' sera.
Conclusions:
- Anti-human CD4 monoclonal antibodies are effective tools for T-lymphocyte identification in Cynomolgus monkeys.
- The described methods provide valuable techniques for primate immunology research.
- These techniques facilitate further investigation into immune function in non-human primates.