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Profiling taxanes in Taxus extracts using lc/ms and lc/ms/ms techniques
E H Kerns1, K J Volk, S E Hill
1Bristol-Myers Squibb Pharmaceutical Research Institute, Analytical Research and Development Department, Wallingford, Connecticut 06492-7660.
Journal of Natural Products
|October 1, 1994
Summary
A new analytical method enables rapid identification of taxanes from Taxus species. This technique uses mass spectrometry to compare taxane structures against a paclitaxel template, creating a valuable database for natural product analysis.
Area of Science:
- Natural Product Chemistry
- Analytical Chemistry
- Mass Spectrometry
Background:
- Taxanes are a class of natural products with significant pharmaceutical applications.
- Accurate and efficient structure elucidation of taxanes from complex mixtures is challenging.
Purpose of the Study:
- To develop and apply an integrated analytical methodology for the rapid and systematic structure elucidation of taxanes.
- To establish a taxane profile database for identifying taxanes in crude extracts and process intermediates.
Main Methods:
- Integration of High-Performance Liquid Chromatography (HPLC) with UV detection and spectroscopy.
- On-line full-scan ionspray mass spectrometry (MS) and tandem mass spectrometry (MS/MS).
- Structure identification based on comparing mass spectrometric characteristics to a paclitaxel substructural template.
Main Results:
- The methodology was applied to analyze taxanes from Taxus brevifolia and Taxus baccata.
- Comprehensive analytical data, including chromatographic characteristics, molecular weight, and MS/MS product ion spectra, were obtained for 18 taxanes.
- A database of taxane profiles was generated.
Conclusions:
- The developed analytical method allows for rapid and systematic structure elucidation of taxanes.
- The taxane profile database facilitates the identification of taxanes in limited quantity samples and complex mixtures.
- This approach enhances the analysis of natural products for drug discovery and development.