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Related Experiment Videos

Ca(2+)-activated K+ channels are involved in regulatory volume decrease in acinar cells isolated from the rat

K P Park1, J S Beck, I J Douglas

  • 1Cell Physiology and Renal Research Groups, School of Biological Sciences, University of Manchester, United Kingdom.

The Journal of Membrane Biology
|August 1, 1994
PubMed
Summary

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Rat lacrimal gland cells use calcium-activated potassium channels to regulate cell volume. These channels facilitate potassium loss during regulatory volume decrease (RVD) in hypotonic solutions.

Area of Science:

  • Cell physiology
  • Ion channel function
  • Epithelial transport

Background:

  • Lacrimal gland acinar cells regulate their volume in response to osmotic stress.
  • Regulatory volume decrease (RVD) is a critical cellular process for maintaining homeostasis.
  • The specific ion channels involved in RVD in lacrimal gland cells are not fully elucidated.

Purpose of the Study:

  • To investigate the role of ion channels in regulatory volume decrease (RVD) in rat lacrimal gland acinar cells.
  • To identify the types of potassium channels involved in RVD.
  • To determine the dependence of RVD on extracellular calcium and potassium channels.

Main Methods:

  • Video-imaging was used to measure cell volumes of isolated rat lacrimal gland acinar cells.

Related Experiment Videos

  • Cell-attached and inside-out patch-clamp electrophysiology were employed to study ion channel activity.
  • The effects of hypotonic solutions, extracellular calcium, and tetraethylammonium chloride (TEA+) on cell volume and channel activity were assessed.

    • Main Results:

    • Rat lacrimal gland acinar cells exhibited RVD when exposed to hypotonic solutions.
    • RVD was inhibited by the absence of extracellular calcium and by the potassium channel blocker TEA+.
    • Hypotonic stress activated maxi calcium-activated potassium channels (maxi KCa channels) with a conductance of 141 ± 6 pS.
    • These maxi KCa channels were partially blocked by TEA+ and their activation was dependent on extracellular calcium.

    Conclusions:

    • Maxi calcium-activated potassium channels play a significant role in mediating regulatory volume decrease (RVD) in rat lacrimal gland acinar cells.
    • The opening of these channels facilitates potassium efflux, contributing to cell volume regulation.
    • Extracellular calcium is essential for the activation of these channels during RVD.