Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Universal probe system for DNA fingerprinting

Y Tamaki1, T Kishida, M Fukuda

  • 1Department of Forensic Medicine, Oita Medical University, Japan.

Nihon Hoigaku Zasshi = the Japanese Journal of Legal Medicine
|October 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Poly-N-acetyllactosaminyl O-glycans attached to leukosialin. The presence of sialyl Le(x) structures in O-glycans.

The Journal of biological chemistry·1992
Same author

Shortening of N1 and P3 latencies in event-related potentials observed coincidentally with clinical improvement during nootropic medication in a demented patient: specific effect of nicergoline.

The Japanese journal of psychiatry and neurology·1992
Same author

Chediak-Higashi lymphoblastoid cell lines: granule characteristics and expression of lysosome-associated membrane proteins.

Clinical immunology and immunopathology·1992
Same author

Distributions of the Nd and P300 in a normal sample.

International journal of psychophysiology : official journal of the International Organization of Psychophysiology·1992
Same author

[Short-latency somatosensory evoked potentials in patients with hypertensive intracerebral hemorrhage: clinical availability of N20 component].

No shinkei geka. Neurological surgery·1992
Same author

[Clinical characteristic of elderly patients with insulin-dependent diabetes mellitus].

Nihon Ronen Igakkai zasshi. Japanese journal of geriatrics·1992
Same journal

[Abstracts of the 99th Congress of the Japanese Society of Legal Medicine. June 10-12, 2015, Kouchi, Japan].

Nihon hoigaku zasshi = The Japanese journal of legal medicine·2015
Same journal

[Abstract of the branch meetings of the Japanese Society of Legal Medicine. 2013, Japan].

Nihon hoigaku zasshi = The Japanese journal of legal medicine·2014
Same journal

[Abstracts of the 97th Congress of the Japanese Society of Legal Medicine. June 26-28, 2013, Sapporo, Japan].

Nihon hoigaku zasshi = The Japanese journal of legal medicine·2013
Same journal

[Abstract of the branch meetings of the Japanese Society of Legal Medicine. 2012, Japan].

Nihon hoigaku zasshi = The Japanese journal of legal medicine·2013
Same journal

[Abstract of the branch meetings of the Japanese Society of Legal Medicine. 2010, Japan].

Nihon hoigaku zasshi = The Japanese journal of legal medicine·2012
Same journal

[Resident training program of coroner's office and trauma quality assurance in Los Angeles County, California, USA].

Nihon hoigaku zasshi = The Japanese journal of legal medicine·2011
See all related articles

This study introduces a universal probe system for DNA fingerprinting, saving time and labor by using a labeled secondary probe with unlabeled primary probes in a cloning vector. The system successfully detected DNA fingerprints in human samples.

Area of Science:

  • Molecular Biology
  • Genetics

Background:

  • Traditional DNA probe labeling is time-consuming and labor-intensive.
  • A universal probe system offers a more efficient alternative for repetitive labeling tasks.

Purpose of the Study:

  • To develop and validate a universal probe system for DNA fingerprinting applications.
  • To demonstrate the efficacy of using unlabeled primary probes with labeled secondary probes.

Main Methods:

  • Isolation of multi-locus probes from human myeloma DNA subgenomic library inserted into phagemid pUC118.
  • Preparation of single-stranded DNA from recombinant phagemids.
  • Hybridization of probes to Southern blots of DNA from mother-child-father trios and unrelated individuals.

Main Results:

Related Experiment Videos

  • Successful detection of DNA fingerprints using the universal probe system.
  • Demonstrated comparability to traditional double-stranded DNA probes.
  • Effectiveness shown with both alkaline phosphatase- and digoxigenin-labeled secondary probes.

Conclusions:

  • The developed universal probe system is effective for DNA fingerprinting.
  • This method significantly reduces the time and labor associated with probe preparation.
  • The system provides a versatile and efficient tool for genetic analysis.