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Related Experiment Videos

CNTF receptor alpha mRNA expression in rodent cell lines and developing rat

A J MacLennan1, A A Gaskin, D C Lado

  • 1Department of Neuroscience, University of Florida College of Medicine, Gainesville 32610.

Brain Research. Molecular Brain Research
|September 1, 1994
PubMed
Summary

Ciliary neurotrophic factor (CNTF) influences neuronal development by binding to its receptor alpha component (CNTFR alpha). CNTFR alpha mRNA is widely expressed in the brain, suggesting a crucial role in neuronal maintenance.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Developmental Biology

Background:

  • Ciliary neurotrophic factor (CNTF) regulates neuronal survival, proliferation, and differentiation.
  • CNTF exerts its effects primarily through binding to the CNTF receptor alpha component (CNTFR alpha).

Purpose of the Study:

  • To investigate the expression patterns of CNTFR alpha mRNA during rat development.
  • To determine the cellular distribution of CNTFR alpha mRNA in various rodent cell lines.
  • To explore potential cross-talk between neurotrophin and CNTF signaling pathways.

Main Methods:

  • Cloning of a partial rat CNTFR alpha cDNA.
  • Northern blot analysis to detect CNTFR alpha mRNA expression in different tissues and developmental stages.
  • Analysis of CNTFR alpha mRNA levels in various rodent cell lines, including neuronal, Schwann cell, fibroblast, and glioma lines.

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  • Treatment of PC12 cells with nerve growth factor to assess effects on CNTFR alpha mRNA levels.

    • Main Results:

    • CNTFR alpha mRNA is predominantly expressed in the brain of embryonic and postnatal rats.
    • CNTFR alpha transcripts are detected throughout brain development, from embryonic day 12 to adulthood, with widespread distribution in the adult brain.
    • Highest CNTFR alpha mRNA concentrations were found in neuronal cell lines, with lower levels in a Schwann cell line and undetectable levels in fibroblast and glioma lines.
    • Nerve growth factor treatment led to a decrease in CNTFR alpha mRNA levels in PC12 cells.

    Conclusions:

    • CNTFR alpha mRNA expression is primarily localized to the brain and is present throughout development.
    • The expression profile suggests a significant role for CNTFR alpha in the central nervous system.
    • Cross-talk between neurotrophin and CNTF signaling pathways, as evidenced by nerve growth factor's effect on CNTFR alpha mRNA, may be critical for neuronal development and maintenance.