Related Experiment Video
Updated: Aug 9, 2026

Generation of Stable Human Cell Lines with Tetracycline-inducible (Tet-on) shRNA or cDNA Expression
Published on: March 5, 2013
Genetic selection of growth-inhibitory sequences in mammalian cells
1Department of Genetics, University of Illinois College of Medicine, Chicago 60612.
Abstract:
To assess the role of mitogenically activated genes in the control of cell proliferation, we have taken a genetic approach based on the premise that blocking the function of an essential gene should lead to growth inhibition. Using a newly developed selection procedure, we isolated growth-inhibitory sequences from a pool of random cDNA fragments of 19 growth-related genes associated with the G0/G1 transition. These sequences encode potential dominant negative variants of c-Fos, JunB, and p44MAPK that may interfere with their growth-related functions. We anticipate that this procedure, which allows for the selection of sequences that cause a growth-inhibition phenotype, may have broad applications in the identification and analysis of genes that regulate cell growth.
More Related Videos
Related Concept Videos
Negative Regulator Molecules
In-vitro Mutagenesis
Eukaryotic Transcription Inhibitors
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
Inhibition of Cdk Activity
Abnormal Proliferation

