Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Altered striatal function in a mutant mouse lacking D1A dopamine receptors

J Drago1, C R Gerfen, J E Lachowicz

  • 1Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.

Proceedings of the National Academy of Sciences of the United States of America
|December 20, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An observational study of farmer-reported clinical mastitis in New Zealand dairy ewes.

New Zealand veterinary journal·2024
Same author

Volumetric MRI Study of the Brain in Fetuses with Intrauterine Cytomegalovirus Infection and Its Correlation to Neurodevelopmental Outcome.

AJNR. American journal of neuroradiology·2019
Same author

Molecular detection of Bartonella coopersplainsensis and B. henselae in rats from New Zealand.

New Zealand veterinary journal·2018
Same author

The Effect of Urine Concentration and pH on the Growth of Escherichia Coli in Canine Urine In Vitro.

Journal of veterinary internal medicine·2018
Same author

Characterisation of extended-spectrum β-lactamase and AmpC β-lactamase-producing Enterobacteriaceae isolated from companion animals in New Zealand.

New Zealand veterinary journal·2016
Same author

Subclinical Bacteriuria in Older Cats and its Association with Survival.

Journal of veterinary internal medicine·2016

Mice lacking dopamine D1A receptors show altered striatal neuron function, impacting the direct pathway crucial for movement regulation. These findings offer insights into dopamine receptor roles in neurological conditions.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Dopamine receptors D1A and D2 are key in the adult brain striatum.
  • These receptors are differentially distributed in direct and indirect neuronal pathways.
  • Imbalances in these pathways are linked to movement disorders like Parkinson's disease.

Purpose of the Study:

  • To investigate the interaction between D1A and D2 neuronal pathways in the striatum.
  • To characterize the phenotype of mice lacking functional D1A receptors.

Main Methods:

  • Homologous recombination was used to generate D1A receptor-deficient (D1A-/-) mice.
  • Phenotypic analysis included growth, survival, locomotion, and rearing behavior assessments.
  • Striatal tissue was examined for D1A receptor binding, neuronal persistence, and mRNA levels (substance P, enkephalin).

Related Experiment Videos

Main Results:

  • D1A-/- mice require dietary supplementation for survival and exhibit reduced rearing behavior.
  • Absence of D1A receptor binding was confirmed in D1A-/- striatum.
  • Striatal neurons expressing D1A receptors persisted, but substance P mRNA levels were reduced, while enkephalin mRNA levels remained unaffected.

Conclusions:

  • D1A receptor deficiency leads to selective functional alterations in striatal neurons of the direct output pathway.
  • This study highlights the specific role of D1A receptors in regulating the direct striatal pathway.
  • Findings contribute to understanding dopamine receptor function in movement control and related disorders.