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Related Experiment Videos

Sense antisense DNA strand?

Z Boldogkói1, A V Kaliman, J Murvai

  • 1Institute of Biochemistry and Protein Research, Agricultural Biotechnology Center, Gödöllö, Hungary.

Acta Veterinaria Hungarica
|January 1, 1994
PubMed
Summary
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Alphaherpesviruses express latency-associated transcripts (LATs) from immediate-early (IE) genes. However, high GC content in herpesvirus DNA suggests that extended open reading frames (ORFs) in LATs may not indicate actual protein translation.

Area of Science:

  • Virology
  • Molecular Biology
  • Genomics

Background:

  • Alphaherpesviruses are known to express latency-associated transcripts (LATs).
  • These LATs originate from the antisense strand of immediate-early (IE) genes.
  • It has been hypothesized that LATs with extended open reading frames (ORFs) could be translated into proteins.

Purpose of the Study:

  • To investigate the potential for protein production from herpesvirus latency-associated transcripts (LATs).
  • To analyze the genetic features of herpesvirus DNA that might influence translation.
  • To determine if extended open reading frames (ORFs) in LATs are indicative of actual protein synthesis.

Main Methods:

  • Analysis of DNA sequences from various herpesviruses.
  • Examination of codon usage bias, specifically GC preference at the third codon position.

Related Experiment Videos

  • Assessment of the probability of stop-codon occurrence within different reading frames.

    • Main Results:

    • A significant GC preference at the third codon position was observed in the DNA of certain herpesviruses.
    • This GC bias drastically reduces the likelihood of stop codons appearing in two of the six possible DNA reading frames.
    • The presence of extended ORFs in LATs is a consequence of this GC bias, not necessarily a sign of active translation.

    Conclusions:

    • The high GC content in herpesvirus DNA influences ORF prediction.
    • Extended ORFs in latency-associated transcripts (LATs) may be artifacts of codon bias.
    • The findings suggest that LATs with extended ORFs are unlikely to be translated into functional proteins.