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Updated: Aug 12, 2026

Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae
Published on: June 3, 2017
Alterations of binding mode and cutting site by G-->I replacement in preferred cleavage sequences 5'-AGG of
1Institute for Chemical Research, Kyoto University, Japan.
Abstract:
Replacement of guanosine by inosine at the center position of a target trinucleotide 5'-AGG resulted in greatly diminished DNA cleavage by C-1027 chromophore, indicating that the guanine 2-amino group significantly participates to DNA binding and cleavage by the chromophore of C-1027. This notion was supported by fluorescence titrations of the chromophore with [poly(dG-dC)]2 and [poly(dI-dC)]2. In preferred cleavage sequence 5'-AGG of the enediyne chromophore of C-1027, the G-->I substitution clearly induces change of DNA binding mode and cutting site.
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