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Related Experiment Videos

The alkaline phosphatase in human plexus chorioideus

Y Nishihara1, Y Hayashi, T Fujii

  • 1Department of Pharmaceutics, Gifu Pharmaceutical University, Japan.

Biochimica Et Biophysica Acta
|December 14, 1994
PubMed
Summary

Alkaline phosphatase isozymes are low in most human brain tissues but abundant in the choroid plexus. This tissue-specific alkaline phosphatase is the same type found in the liver.

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Area of Science:

  • Biochemistry
  • Neuroscience
  • Enzymology

Background:

  • Alkaline phosphatase (AP) isozymes are crucial enzymes with diverse physiological roles.
  • Their distribution and function in human brain tissues are not fully understood.
  • Previous studies have focused on AP isozymes in peripheral organs.

Purpose of the Study:

  • To investigate the presence and characteristics of alkaline phosphatase isozymes in various human brain regions.
  • To identify the specific AP isozyme expressed in the human choroid plexus.
  • To explore the potential functional implications of AP in the brain.

Main Methods:

  • Monoclonal immunocatalytic assays were employed to quantify AP isozyme content.
  • Human brain tissues and other human tissues (liver, kidney, bone, intestine, placenta) were analyzed.

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  • Purified isozymes were characterized using SDS-polyacrylamide gel electrophoresis and reactivity with specific monoclonal antibodies.

    • Main Results:

    • AP isozyme levels were generally low in most human brain regions compared to other tissues.
    • Significant AP activity was detected in the human choroid plexus.
    • The AP isozyme from the choroid plexus was identified as tissue-unspecific alkaline phosphatase (TNSALP), sharing characteristics with liver AP.

    Conclusions:

    • The choroid plexus is a major site of AP activity within the human brain.
    • TNSALP is expressed in the human choroid plexus, suggesting a specific role in this brain structure.
    • This finding expands the understanding of AP isozymes' functional significance in the central nervous system.