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Related Experiment Videos

Dynamic gap junctional communication: a delimiting model for tissue responses

G J Christ1, P R Brink, S V Ramanan

  • 1Department of Urology, Albert Einstein College of Medicine, Bronx, New York 10461.

Biophysical Journal
|September 1, 1994
PubMed
Summary
This summary is machine-generated.

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Second messenger molecules dynamically regulate intercellular communication through gap junctions. This study models how transient changes in these molecules alter cell coupling, impacting tissue function.

Area of Science:

  • Cellular Biology
  • Biophysics
  • Physiology

Background:

  • Gap junctions, formed by connexins, facilitate intercellular communication via aqueous channels.
  • These channels are permeable to second messenger molecules (e.g., Ca2+, IP3, cAMP, cGMP) that influence junctional patency.
  • The precise modulation of intercellular communication by transient second messenger changes remains unclear.

Purpose of the Study:

  • To develop a modified tissue monolayer model to investigate the dynamic regulation of gap junction communication.
  • To account for both up-regulatory and down-regulatory effects of second messengers on gap junctions.
  • To explore gap junction behavior in the vascular wall, a tissue rich in these junctions.

Main Methods:

  • Modification of the Ramanan and Brink (1990) tissue monolayer model.

Related Experiment Videos

  • Incorporation of second messenger diffusion through gap junctions.
  • Simulation of gap junction dynamics under physiologically relevant conditions, focusing on the vascular wall.

    • Main Results:

    • Modeling demonstrated that transient changes in intracellular second messenger concentrations can induce significant alterations in intercellular communication.
    • A 50-125% change in the number of cells within a functional syncytium was observed after single-cell activation.
    • The model conditions for these changes are consistent with observations in intact and cultured tissues.

    Conclusions:

    • Dynamic changes in second messenger concentrations play a crucial role in modulating intercellular communication extent.
    • This model provides a framework for understanding how transient molecular signals impact cell-to-cell communication networks.
    • The findings have implications for understanding tissue function and disease states involving altered gap junction activity.