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Published on: November 15, 2017
Partial sequencing and mapping of clones from two maize cDNA libraries
B Shen1, N Carneiro, I Torres-Jerez
1Dept. of Plant Sciences, University of Arizona, Tucson 85721.
Plant Molecular Biology
|November 1, 1994
Summary
Researchers analyzed maize complementary DNAs (cDNAs) using gene expression, sequencing, and mapping. This approach helps identify gene functions within the maize genome project.
Area of Science:
- Genomics
- Molecular Biology
- Plant Science
Background:
- Maize genome project requires comprehensive analysis of complementary DNAs (cDNAs).
- Understanding gene expression patterns and functions is crucial for maize genetic research.
Purpose of the Study:
- To analyze randomly selected maize cDNAs using a combination of mRNA expression analysis, single-pass sequencing (SPS), and genome mapping.
- To categorize cDNA clones based on transcription levels and tissue specificity.
- To determine the chromosomal location of cDNA clones.
Main Methods:
- Analysis of etiolated seedling (490) and endosperm cDNA clones (576).
- Measurement of mRNA expression levels via hybridization.
- Single-pass sequencing (SPS) of 313 clones and comparison with GenBank database.
- Restriction Fragment Length Polymorphism (RFLP) mapping for chromosomal localization of over 300 clones.
Main Results:
- cDNA clones were categorized as abundantly or rarely expressed, and as constitutive or tissue-specific.
- Approximately 61% of sequenced clones showed no significant similarity in GenBank, 14% showed high similarity, and 25% showed lower similarity.
- Chromosomal locations were determined for over 300 cDNA clones using RFLP mapping.
Conclusions:
- A combined approach of mRNA expression, sequencing, and genome mapping provides synergistic data for functional genomics.
- This study contributes to the characterization of the maize genome by analyzing a set of randomly selected cDNAs.
- The findings facilitate the eventual deduction of functions for unknown cDNA clones within the maize genome.
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