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Molecular analysis of two Brassica napus genes expressed in the stigma
L S Robert1, S Allard, J L Gerster
1Plant Research Centre, Central Experimental Farm, Ottawa, Ontario, Canada.
Plant Molecular Biology
|November 1, 1994
Abstract:
A partial cDNA clone, Pis 63, corresponding to a mRNA highly expressed in Brassica napus pistils, was isolated by differential screening. PCR was used to complete the Pis 63 sequence (Pis 63-1) and to obtain the sequence of another related cDNA (Pis 63-2). Northern blot and in situ analyses demonstrated that these transcripts are expressed in the stigma throughout flower development. Pis 63-1 and Pis 63-2 display similarity to a cotton fibre cDNA clone.