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Expression of the complement regulator factor C8 binding protein on human glomerular cells protects them from
G Schieren1, M Schönermark, M Braunger
1Institut für Immunologie, Universität Heidelberg, Deutschland.
Experimental Nephrology
|September 1, 1994
Summary
The complement-regulatory factor C8 binding protein (C8bp) protects human glomerular cells from complement-mediated damage. Blocking C8bp function increases cell susceptibility to complement attack, highlighting its role in cellular defense.
Area of Science:
- Immunology
- Cell Biology
- Renal Physiology
Background:
- C8 binding protein (C8bp) is a complement-regulatory factor identified on erythrocyte membranes.
- C8bp inhibits complement component C8 and prevents homologous complement-mediated lysis.
Purpose of the Study:
- To investigate the expression and function of C8bp in human glomerular cells.
- To determine C8bp's role in cellular defense against complement attack.
Main Methods:
- Cultured human glomerular mesangial and epithelial cells (GEC) were used.
- C8bp surface expression was analyzed after stimulation with IL-1β, C5b-9, or endotoxin.
- Cellular susceptibility to complement-mediated killing was assessed after blocking C8bp function with an anti-C8bp antibody fragment.
Main Results:
- C8bp was expressed on the surface and in the cytoplasm of cultured GEC.
- Stimulation increased C8bp surface expression transiently and independently of protein synthesis.
- Blocking C8bp function made GEC susceptible to complement-mediated killing.
Conclusions:
- C8bp is expressed in human glomerular cells and contributes to their defense.
- C8bp plays a protective role against complement-mediated damage in the glomerulus.