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Related Experiment Videos

Glucose attenuates a morphine-induced decrease in hippocampal acetylcholine output: an in vivo microdialysis study in

M E Ragozzino1, G L Wenk, P E Gold

  • 1Department of Psychology, University of Virginia, Charlottesville 22903.

Brain Research
|August 29, 1994
PubMed
Summary

Glucose administration may reverse morphine-induced memory impairments by restoring acetylcholine levels in the brain. This study investigated how glucose affects acetylcholine output after morphine treatment.

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Area of Science:

  • Neuroscience
  • Pharmacology

Background:

  • Systemic morphine impairs memory performance.
  • Glucose administration can ameliorate these morphine-induced memory deficits.
  • This effect may involve the modulation of acetylcholine release by glucose.

Purpose of the Study:

  • To determine if systemic morphine decreases acetylcholine output in the hippocampal formation.
  • To investigate if systemic glucose administration attenuates morphine's effect on acetylcholine output.

Main Methods:

  • Microdialysis was used to collect samples from the hippocampal formation at 12-min intervals.
  • Acetylcholine levels were measured using High-Performance Liquid Chromatography (HPLC) with electrochemical detection.
  • Morphine (10 mg/kg) and glucose (100 mg/kg) were administered systemically.

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Main Results:

  • Morphine injections caused an immediate 20-35% decrease in acetylcholine output, lasting up to 36 minutes.
  • Concurrent glucose administration attenuated this reduction in acetylcholine output in subsequent samples.
  • These findings indicate a direct effect of glucose on morphine's impact on acetylcholine.

Conclusions:

  • Systemic morphine treatment decreases acetylcholine output in the hippocampus.
  • Glucose administration can mitigate this morphine-induced decrease in acetylcholine.
  • Glucose may attenuate morphine-induced memory impairments by restoring hippocampal acetylcholine levels.