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Human cyclin F

C Bai1, R Richman, S J Elledge

  • 1Howard Hughes Medical Institute, Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030.

The EMBO Journal
|December 15, 1994
PubMed
Summary
This summary is machine-generated.

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Researchers discovered a new human protein, cyclin F, crucial for cell cycle regulation. This protein plays a key role in coordinating cell division events, distinct from other known cyclins.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Cyclins are key regulators of cell cycle transitions by activating cyclin-dependent protein kinases.
  • Mammalian cells utilize various cyclins to coordinate cell cycle progression.
  • The precise roles of all cyclins in cell cycle control are not fully elucidated.

Purpose of the Study:

  • To identify and characterize a novel human cyclin, designated cyclin F.
  • To investigate the function of cyclin F in cell cycle regulation.
  • To understand the expression pattern and localization of cyclin F.

Main Methods:

  • Isolation of human cyclin F as a suppressor of a yeast cell cycle mutant (cdc4).
  • Analysis of cyclin F molecular weight, protein domains (PEST-rich C-terminus, cyclin box), and mRNA expression.

Related Experiment Videos

  • Investigation of cyclin F protein accumulation, degradation timing, and subcellular localization (nucleus, perinuclear).
  • Functional studies involving overexpression of cyclin F and a mutant lacking the PEST region in human cells.

    • Main Results:

    • Cyclin F is the largest known cyclin (87 kDa), with a PEST-rich C-terminus and a cyclin box similar to cyclins A and B.
    • Cyclin F mRNA is ubiquitously expressed and its levels fluctuate during the cell cycle, peaking in G2.
    • Cyclin F protein accumulates during interphase and is degraded at mitosis, with distinct subcellular localization.
    • Overexpression of cyclin F leads to an increased G2 cell population.

    Conclusions:

    • Cyclin F is a novel, ubiquitously expressed human cyclin involved in cell cycle regulation.
    • Its unique expression, localization, and cell cycle-dependent degradation suggest a distinct role from other cyclins.
    • Cyclin F likely coordinates essential cell cycle events, contributing to the overall control of cell division.