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Related Experiment Videos

IS231 and other Bacillus thuringiensis transposable elements: a review

J Mahillon1, R Rezsöhazy, B Hallet

  • 1Unité de Génétique, Université Catholique de Louvain, Louvain-La-Neuve, Belgium.

Genetica
|January 1, 1994
PubMed
Summary

Bacillus thuringiensis delta-endotoxin genes are linked to mobile genetic elements like insertion sequences (IS) and transposons. These elements facilitate gene mobility, influencing bacterial toxicity and pest control applications.

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Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Bacillus thuringiensis produces delta-endotoxin crystals toxic to pests.
  • Toxin genes are often plasmid-borne and associated with mobile genetic elements.
  • Mobile elements like insertion sequences (IS) and transposons are linked to toxin gene mobility.

Purpose of the Study:

  • To investigate the role of insertion sequences and transposons in Bacillus thuringiensis toxin gene mobility.
  • To analyze the structural and functional characteristics of these mobile elements.
  • To understand the mechanisms of DNA-protein interactions during transposition.

Main Methods:

  • Structural analysis of iso-IS231 elements.
  • Functional assays of IS231A transposition in Escherichia coli, Bacillus subtilis, and B. thuringiensis.

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  • Comparative analysis with related insertion sequence families.

    • Main Results:

    • Iso-IS231 elements show structural relationships with IS1151 and IS4 family members.
    • A conserved transposase-integrase motif was identified.
    • IS231A demonstrated a non-replicative transposition mode with target site preference in multiple bacterial species.

    Conclusions:

    • Mobile genetic elements, particularly insertion sequences, play a crucial role in the mobility of Bacillus thuringiensis toxin genes.
    • Understanding these elements aids in comprehending bacterial toxicity variation.
    • A model for DNA-protein interactions at transposition target sites is proposed.