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Evaluation of BBL crystal MRSA ID system
C C Knapp1, M D Ludwig, J A Washington
1Cleveland Clinic Foundation 44195.
Journal of Clinical Microbiology
|October 1, 1994
Summary
The BBL Crystal system accurately identifies methicillin-resistant Staphylococcus aureus (MRSA) by detecting the mecA gene. This diagnostic tool shows high correlation with mecA gene presence in Staphylococcus aureus and Staphylococcus epidermidis isolates.
Area of Science:
- Clinical Microbiology
- Infectious Diseases
- Diagnostic Technology
Background:
- Accurate detection of methicillin resistance in Staphylococcus species is crucial for effective treatment.
- The BBL Crystal system is a diagnostic tool for identifying methicillin-resistant Staphylococcus aureus (MRSA).
Purpose of the Study:
- To evaluate the BBL Crystal system's accuracy in identifying oxacillin resistance in Staphylococcus aureus.
- To explore its utility in detecting oxacillin resistance in Staphylococcus epidermidis.
Main Methods:
- Testing of Staphylococcus aureus and Staphylococcus epidermidis isolates with known mecA gene status.
- Evaluation of the BBL Crystal system's MRSA ID test reaction at 4, 5, and 6 hours.
Main Results:
- High correlation observed between mecA gene positivity and positive BBL Crystal MRSA ID test results for Staphylococcus aureus (up to 99% agreement).
- The system demonstrated good agreement for mecA gene-positive Staphylococcus epidermidis isolates (up to 96% agreement).
- All mecA gene-negative staphylococci yielded negative MRSA ID test reactions.
Conclusions:
- The BBL Crystal system is a reliable tool for identifying oxacillin resistance in Staphylococcus aureus.
- The system shows potential utility for detecting oxacillin resistance in Staphylococcus epidermidis.