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Rapid detection of vancomycin-resistant enterococci

S C Edberg1, C J Hardalo, C Kontnick

  • 1Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06510.

Journal of Clinical Microbiology
|September 1, 1994
PubMed
Summary
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This study presents an inexpensive method to detect vancomycin-resistant enterococci using a selective agar medium and rapid biochemical tests. This approach allows for quick and accurate identification of these resistant bacteria in clinical specimens.

Area of Science:

  • Clinical microbiology
  • Bacteriology
  • Antimicrobial resistance

Background:

  • Enterococci are common causes of hospital-acquired infections.
  • Vancomycin resistance in enterococci (VRE) is a significant public health concern.
  • Rapid and accurate detection of VRE is crucial for infection control.

Purpose of the Study:

  • To develop an inexpensive and rapid method for screening and identifying vancomycin-resistant enterococci (VRE).
  • To evaluate the efficacy of a selective culture medium combined with biochemical tests for VRE detection.

Main Methods:

  • Campylobacter blood agar supplemented with clindamycin was used as a selective medium.
  • Incubation was performed under 6% CO2.
  • Presumptive enterococci colonies were identified using pyroglutamyl-beta-naphthylamide and rapid bile esculin tests.

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Main Results:

  • The selective medium effectively screened for vancomycin resistance and selected for presumptive enterococci.
  • Enterococci were specifically identified within 30 minutes using the enzyme substrate tests.
  • The combined method proved to be an inexpensive approach for enumerating VRE.

Conclusions:

  • The combination of a selective medium and rapid biochemical tests provides an efficient and cost-effective method for detecting vancomycin-resistant enterococci.
  • This method facilitates the rapid enumeration of VRE from clinical specimens using readily available reagents.
  • This approach can aid in timely infection control measures against VRE.