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Effect of potential interference factors on performance of enzyme immunoassay and latex agglutination assay for

H D Engler1, Y R Shea

  • 1Clinical Pathology Department, Warren Grant Magnuson Clinical Center, Bethesda, Maryland 20892.

Journal of Clinical Microbiology
|September 1, 1994
PubMed

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.