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VASE exon expression alters NCAM-mediated cell-cell interactions

A Chen1, S Haines, K Maxson

  • 1Division of Basic Research, Children's Hospital Research Foundation, Cincinnati, Ohio 45229-2899.

Journal of Neuroscience Research
|August 1, 1994
PubMed
Summary

Structural variations in neural cell adhesion molecule (NCAM) influence cell behavior. Specifically, the VASE exon impacts how NCAM affects cell aggregation and segregation, demonstrating its role in cell-cell interactions.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cell Biology

Background:

  • Neural cell adhesion molecule (NCAM) exists as multiple polypeptides from alternative splicing of a single gene.
  • The VASE exon is a key structural variation in NCAM, located near cell adhesion domains.

Purpose of the Study:

  • To investigate how distinct NCAM forms, with and without the VASE exon, mediate cell adhesion.
  • To determine if structural differences in NCAM are sufficient to alter cell aggregation and segregation.

Main Methods:

  • Transfection of L cells with expression vectors for rat 140 kD NCAM, with and without the VASE exon.
  • Isolation and testing of transfected L cell lines for self-aggregation in a low shear assay.
  • Assessment of cell segregation using a long-term co-aggregation assay.

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Main Results:

  • Cellular aggregation levels correlated with the specific NCAM molecule expressed by transfectants.
  • Significant segregation was observed: cells expressing NCAM without the VASE exon excluded cells with the VASE exon, and vice versa.

Conclusions:

  • The presence or absence of the VASE exon in NCAM directly influences cell aggregation.
  • Even minor structural changes in NCAM, like the VASE exon, are sufficient to drive cell segregation, highlighting its role in cell-cell interactions.