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Related Experiment Videos

The determination of interfacial transfer constants using side-by-side diffusion cells

G L Mosher1

  • 1INTERx Research Division, Merck and Company, Lawrence, Kansas 66047.

Pharmaceutical Research
|September 1, 1994
PubMed
Summary

Side-by-side diffusion cells accurately measure interfacial transfer constants for diverse compounds. This method provides reliable data for understanding drug transport and formulation.

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Area of Science:

  • Physicochemical Pharmaceutics
  • Biophysical Chemistry
  • Drug Delivery Science

Background:

  • Accurate determination of interfacial transfer constants is crucial for predicting drug behavior in biological systems.
  • Existing methods may lack precision or controlled experimental conditions for interfacial transport studies.
  • Understanding compound partitioning between aqueous and lipid phases is key to drug absorption and distribution.

Purpose of the Study:

  • To evaluate the utility of a side-by-side diffusion cell setup for quantifying interfacial transfer constants.
  • To determine pH 7.4 buffer-octanol interfacial transfer constants for a diverse set of compounds.
  • To validate a resistance summation model for deriving transfer constants from transport data.

Main Methods:

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  • Employed a side-by-side diffusion cell apparatus to measure transport rates across an interface.
  • Investigated twelve compounds including pharmaceuticals, amino acids, and small organics with log K values from -3 to 2.
  • Utilized a resistance summation model to analyze transport data and derive interfacial transfer constants.

    • Main Results:

    • Successfully determined interfacial transfer constants for all twelve compounds under pH 7.4 conditions.
    • The derived constants showed excellent agreement (r² = 0.999) with a published model correlating transfer constants to equilibrium partition coefficients (K).
    • Demonstrated the robustness and reliability of the side-by-side diffusion cell method.

    Conclusions:

    • Side-by-side diffusion cells are a valuable and effective tool for precise measurement of interfacial transfer constants.
    • The validated resistance summation model accurately describes compound transport across the buffer-octanol interface.
    • This methodology offers advantages such as controlled interfacial area and measurable hydrodynamic effects for biophysical studies.