[Specimen collection in dental plaque and oral microbiology]

Plaque sampling is the first but also one of the most important steps in a microbiological examination. Different devices exist to take a sample, each with their own characteristics. Sterile paperpoints and curettes are most commonly used, but select a different flora. Isolation of the sample side is of utmost importance to prevent contamination, because the composition and concentration of the plaque differs from region to region (within the oral cavity but also within the pocket). Samples are collected and diluted in different fluids, of which Reduced Transport Fluid is preferred if an anaerobic culture procedure is planned.