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Distribution of C-CAM in developing oral tissues
A Rass1, C Lüning, J Wroblewski
1Department of Oral Diagnostics, School of Dentistry, Karolinska Institutet, Huddinge, Sweden.
Anatomy and Embryology
|September 1, 1994
Summary
Cell adhesion molecule C-CAM is expressed in mouse craniofacial epithelia during development. Its presence in proliferating tissues suggests a role in histogenesis and organogenesis.
Area of Science:
- Developmental biology
- Molecular biology
- Immunology
Background:
- Cell CAM (C-CAM) is a cell surface glycoprotein involved in cell adhesion.
- C-CAM belongs to the carcinoembryonic antigen (CEA) gene family, a subfamily of the immunoglobulin superfamily.
Purpose of the Study:
- To analyze C-CAM expression during normal and disturbed mouse craniofacial development.
- To investigate the role of C-CAM in histogenesis and organogenesis.
Main Methods:
- Immunohistochemistry and in situ hybridization were used to detect C-CAM expression.
- Developmental disturbances were induced by retinoic acid (RA) treatment in pregnant mice.
- Fetuses were examined at various gestational days (14-18).
Main Results:
- C-CAM expression was first detected at day 16 of gestation and increased with age.
- C-CAM was found in ectodermal and mesodermal epithelia, including oral, respiratory, and vascular tissues.
- While RA treatment altered C-CAM expression patterns in palatal shelves, overall expression did not significantly differ between control and treated groups.
Conclusions:
- C-CAM is present in all proliferating craniofacial epithelia.
- The molecule likely plays a significant role in craniofacial development, histogenesis, and organogenesis.