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A rapid protocol for sexing chick embryos (Gallus g. domesticus)
1Division of Development and Reproduction, Roslin Institute, Midlothian, UK.
Animal Genetics
|October 1, 1994
Abstract:
A method for establishing the sex of chick embryos before the appearance or morphological differences between males and females has been developed. DNA was isolated from 5-7-day-old embryos by proteinase K digestion and subjected to polymerase chain reaction (PCR) amplification with W-chromosome specific primers. Sexing can be achieved within 1 day using as little as 1 ng template DNA.