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Failure of test yolk buffer to decrease antisperm antibodies on sperm

D Katsoff1, J H Check, J Kozak

  • 1University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Camden.

Archives of Andrology
|September 1, 1994
PubMed
Summary
This summary is machine-generated.

Incubating sperm in test yolk buffer (TYB) does not reduce the percentage of sperm bound with antisperm antibodies (ASA). This method was ineffective in reducing IgA or IgG attachment to sperm, contrary to previous claims.

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Area of Science:

  • Reproductive immunology
  • Spermatozoa research
  • Infertility diagnostics

Background:

  • Antisperm antibodies (ASA) can impair male fertility.
  • Previous studies suggested that incubating sperm in test yolk buffer (TYB) could reduce ASA.
  • The efficacy of TYB in reducing sperm-bound ASA requires further investigation.

Purpose of the Study:

  • To evaluate if suspending sperm with ASA in TYB reduces the percentage of ASA-positive sperm.
  • To replicate findings by Lam et al. regarding IgA and IgG reduction.
  • To assess the impact of TYB incubation on naturally bound and artificially coated ASA.

Main Methods:

  • Sperm specimens naturally bound with ASA and donor sperm artificially coated with ASA were used.
  • Sperm were incubated in test yolk buffer (TYB).
  • Post-incubation levels of sperm positive for IgA and IgG attachment were quantified.

Main Results:

  • No significant reduction was observed in the percentage of sperm positive for IgA or IgG attachment.
  • This applied to both naturally bound ASA and artificially coated sperm specimens.
  • Incubation in TYB did not effectively decrease the proportion of ASA-bound sperm.

Conclusions:

  • Test yolk buffer (TYB) incubation is not an effective method for reducing the percentage of sperm bound with antisperm antibodies (ASA).
  • Further studies are needed to determine if TYB incubation can reduce the amount of ASA per sperm.
  • The findings do not support the previous claims of significant ASA reduction using this method.