Importance of the C-terminal part of actin in interactions with calponin

Native, modified or trypsin-truncated actin was used to study the impact of modifying the C-terminal part of actin (the last three amino acids) on interactions with calponin. We used three different techniques to show that these amino acids are essential for the actin-calponin interface. The locations of actin-calponin interaction sites on the actin crystal are discussed in terms of previously reported data.