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Related Experiment Videos

A human orphan calcitonin receptor-like structure

B Flühmann1, R Muff, W Hunziker

  • 1Department of Orthopaedic Surgery, University of Zurich, Switzerland.

Biochemical and Biophysical Research Communications
|January 5, 1995
PubMed
Summary

Researchers identified a novel calcitonin receptor-like protein in the human cerebellum. Its specific ligand and function remain unknown, but it shares similarities with known G protein-coupled receptors.

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Area of Science:

  • Molecular biology
  • Genomics
  • Receptor biology

Background:

  • Calcitonin receptors play crucial roles in various physiological processes.
  • Orphan receptors represent a significant area for novel drug discovery.
  • Understanding receptor families aids in predicting function and identifying ligands.

Purpose of the Study:

  • To identify and characterize a novel calcitonin receptor-like protein.
  • To investigate the potential ligands and signaling pathways associated with this new receptor.
  • To determine the expression pattern and cellular localization of the novel receptor.

Main Methods:

  • Molecular cloning of cDNA from human cerebellum.
  • Amino acid sequence alignment with known receptors.

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  • mRNA expression analysis using Northern blotting.
  • Ligand binding assays in transfected COS-7 cells.
  • Cyclic AMP (cAMP) accumulation assays.

    • Main Results:

    • A novel 461-amino acid calcitonin receptor-like protein with seven transmembrane domains was identified.
    • High amino acid identity was observed with rat orphan calcitonin receptor-like sequences (91%) and human calcitonin receptor (56%).
    • The 5.2 kb mRNA was predominantly expressed in the lung, heart, and kidney.
    • Minimal specific binding of known calcitonin and CGRP peptides was detected in transfected cells.
    • No significant cellular cAMP accumulation was observed upon stimulation with various known peptide hormones.

    Conclusions:

    • A novel calcitonin receptor-like protein has been identified, sharing structural similarities with known G protein-coupled receptors.
    • Despite structural homology, the identified protein does not bind or signal in response to established calcitonin receptor ligands.
    • The specific ligand and physiological role of this orphan receptor require further investigation.