Enzyme kinetics by mid-infrared spectroscopy: beta-fructosidase study by a one-step assay
1Université de la Réunion, Laboratoire de Biochimie, Faculté des Sciences, Saint Denis, Reunion.
Abstract:
An alternate method for enzyme study is proposed. Multidimensional statistical analysis applied on mid-infrared attenuated total reflectance spectra (Cadet et al. (1991) Appl. Spectrosc. 42, 166-172) collected during a kinetic allows a direct and fast quantification of the remaining substrate, as well as a one step enzymatic assay. Furthermore, the combination of these techniques may be used as a structural tool. The method applied to the study of beta-fructosidase is developed in this paper as an example. With appropriate calibration, the method may be extend to any enzyme.
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