[Modification of biochemical changes in developing cultures of chick embryo nerve tissue cultures]

The effects of exogenous ganglioside GM1 (1 microM) from bovine brain on the morphological state and biochemical parameters (creatine kinase, acetylcholinesterase and adenylate cyclase activities as well as the protein, phospholipid and ganglioside content) have been studied in primary cultures of trypsin-treated dissociated cells of chicken embryonic brain. Ganglioside GM1 accelerated the growth and differentiation of cultured cells, increased the phospho- and glycolipid content and stimulated the activity of the enzymes.