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Multi-centre comparison of ABBOTT MATRIX Aero to Pharmacia Standard RAST, Modified RAST and skin puncture tests
D R Ownby1, N F Adkinson, R G Hamilton
1Henry Ford Hospital, Detroit, MI.
European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies
|August 1, 1994
Summary
The ABBOTT MATRIX Aero accurately measures specific IgE antibodies to airborne allergens. This enzyme immunoassay shows high sensitivity and specificity compared to skin tests and RAST, offering efficient allergy diagnosis.
Area of Science:
- Allergy diagnostics
- Immunological assays
Background:
- Specific IgE antibody testing is crucial for diagnosing allergic diseases.
- Existing methods like skin prick tests and RAST (Radioallergosorbent Test) have limitations.
Purpose of the Study:
- To evaluate the performance of the ABBOTT MATRIX Aero system for measuring specific IgE antibodies against airborne allergens.
- To compare the ABBOTT MATRIX Aero with skin puncture tests and RAST procedures.
Main Methods:
- The ABBOTT MATRIX Aero, an enzyme immunoassay, was used to measure specific IgE to 14 airborne allergens from single serum samples.
- Results were compared against skin puncture tests and both Standard and Modified RAST.
Main Results:
- ABBOTT MATRIX Aero demonstrated 89% sensitivity and >92% specificity versus Standard and Modified RAST.
- Sensitivity against skin tests was 71%, surpassing Standard RAST (62%) and Modified RAST (67%).
- In vitro systems showed approximately 90% specificity compared to skin tests.
Conclusions:
- The ABBOTT MATRIX Aero system offers favorable comparison to skin tests and RAST for specific IgE quantification.
- This method requires less hands-on time for measuring multiple allergen-specific IgE antibodies.
