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Membrane-associated tubulin in the rat anterior pituitary and GH3 cells
R Ravindra1, R G Nagele, S A Patel
1Department of Cell Biology, UMDNJ-School of Osteopathic Medicine, Stratford 08084.
Plasma membranes from anterior pituitary lobes of rats and GH3 cells were exposed to monoclonal anti-beta tubulin antibodies or normal mouse IgG, and treated with FITC-labeled anti-mouse IgG. Specimens were visualized with a microscope equipped with epifluorescence optics. Tubulin-specific fluorescence was very prominent in membranes incubated with tubulin antibody and was negligible in membranes incubated with normal mouse IgG or FITC-labeled anti-mouse IgG alone. Two dimensional electrophoresis revealed that 27% of the rat pituitary tubulin is beta-tubulin and 73% is alpha-tubulin; 17% of the GH3 cell tubulin is beta-tubulin and 83% is alpha-tubulin. These results suggest that tubulin is a component of plasma membranes of the rat pituitary and GH3 cells.
Plasma membranes from anterior pituitary lobes of rats and GH3 cells were exposed to monoclonal anti-beta tubulin antibodies or normal mouse IgG, and treated with FITC-labeled anti-mouse IgG. Specimens were visualized with a microscope equipped with epifluorescence optics. Tubulin-specific fluorescence was very prominent in membranes incubated with tubulin antibody and was negligible in membranes incubated with normal mouse IgG or FITC-labeled anti-mouse IgG alone. Two dimensional electrophoresis revealed that 27% of the rat pituitary tubulin is beta-tubulin and 73% is alpha-tubulin; 17% of the GH3 cell tubulin is beta-tubulin and 83% is alpha-tubulin. These results suggest that tubulin is a component of plasma membranes of the rat pituitary and GH3 cells.
